IQ REAL™ Quantitative System
Polymerase chain reaction (PCR) technique has been commonly employed in the aquaculture industry for disease diagnosis and prevention. With the growing demand for a quantitative research tool and requirement for a high throughput screening system, the real-time PCR is receiving more attention and becoming more important in the industry. IQ REAL™ Quantitative System is a real-time PCR based diagnostic system which not only meets the above demands, but also its advanced design provides the shrimp culture industry a more accurate, more sensitive, and more flexible real-time system.
- Company NameGENEREACH BIOTECHNOLOGY CORPORATION
Features:
￭ TaqMan® assay strategy: This system can tolerate higher sample loads than SYBR Green based reaction. Consequently, it is able to detect samples of very low target/shrimp cell ratio.
￭ Multiplex design: This system can quantify up to three targets (FAM, VIC, NED/Cy5) including the pathogen and shrimp genomic nucleic acid in a single reaction. The built-in intenal control prevents any false negative interpretation of results from failed extraction or inhibition.
￭ Absolute and comparative quantitation: This system can quantify the target pathogen by both absolute quantitation method (Qab, target quantity/μl) and comparative quantitation method (Qcp, target quantity/10,000 shrimp cells). The Qcp method can reflect the actual infection level and provide consistent results for a sample within a wide range of concentration.
￭ No standard required for Qcp method: There is no need to make a calibration curve for Qcp method. Since the ΔΔCt strategy is used to interpret the final results, there is no need to generate a standard curve in each run, saving reagents costs in the long run.
(TaqMan® is the trademark licensed under Applied Biosystems.)
￭ TaqMan® assay strategy: This system can tolerate higher sample loads than SYBR Green based reaction. Consequently, it is able to detect samples of very low target/shrimp cell ratio.
￭ Multiplex design: This system can quantify up to three targets (FAM, VIC, NED/Cy5) including the pathogen and shrimp genomic nucleic acid in a single reaction. The built-in intenal control prevents any false negative interpretation of results from failed extraction or inhibition.
￭ Absolute and comparative quantitation: This system can quantify the target pathogen by both absolute quantitation method (Qab, target quantity/μl) and comparative quantitation method (Qcp, target quantity/10,000 shrimp cells). The Qcp method can reflect the actual infection level and provide consistent results for a sample within a wide range of concentration.
￭ No standard required for Qcp method: There is no need to make a calibration curve for Qcp method. Since the ΔΔCt strategy is used to interpret the final results, there is no need to generate a standard curve in each run, saving reagents costs in the long run.
(TaqMan® is the trademark licensed under Applied Biosystems.)